Mentions légales
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| 22.08.2009 | |||
| 16:00 | Retford Utd | 1-1 | United of Manchester |
| 16:00 | Stocksbridge PS | 5-3 | Nantwich Town |
| 16:00 | Whitby Town | 3-1 | Frickley Athletic |
| 19.08.2009 | |||
| 20:45 | Whitby Town | 1-2 | Guiseley AFC |
| 20:45 | Worksop Town | 0-0 | Nantwich Town |
| 18.08.2009 | |||
| 20:45 | Burscough | 2-0 | Hucknall Town |
| 20:45 | Marine | 1-1 | Buxton FC |
| 20:45 | Matlock Town | 3-2 ANL | King's Lynn |
| 20:45 | Ossett Town | 4-1 | Frickley Athletic |
| 20:45 | Retford Utd | 1-1 | North Ferriby Utd |
| 20:45 | Stocksbridge PS | 6-0 | Durham City |
| 17.08.2009 | |||
| 20:45 | Ashton United | 2-3 | Kendal Town |
| 20:45 | Bradford PA | 3-2 | United of Manchester |
| 15.08.2009 | |||
| 16:00 | Buxton FC | 1-1 | Bradford PA |
| 16:00 | Durham City | 0-1 | Ashton United |
| 16:00 | Frickley Athletic | 1-0 | Burscough |
| 16:00 | Guiseley AFC | 2-0 | Matlock Town |
| 16:00 | Hucknall Town | 3-0 | Ossett Town |
| 16:00 | Kendal Town | 4-1 | Worksop Town |
| 16:00 | King's Lynn | 1-3 ANL | Stocksbridge PS |
| 16:00 | Nantwich Town | 3-2 | Whitby Town |
| 16:00 | North Ferriby Utd | 0-1 | Marine |
| 16:00 | United of Manchester | 1-2 | Boston United |